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primary antibodies against jak2 3230  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc primary antibodies against jak2 3230
    Primary Antibodies Against Jak2 3230, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies against jak2 3230/product/Cell Signaling Technology Inc
    Average 90 stars, based on 1 article reviews
    primary antibodies against jak2 3230 - by Bioz Stars, 2026-06
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    Dnmt3a expression is repressed by Stat5a and Stat5a is the upstream regulator of Dnmt3a. A. The expression levels of p-STAT5a and DNMT3a proteins in human myeloid tumor cell lines. Full-length blot images are presented in Supplementary Fig. . B. The expression levels of p-Stat5a and Dnmt3a proteins in murine myeloid tumor cell lines. Full-length blot images are presented in Supplementary Fig. . C. In wild type BaF3 cells, the protein expression level of Dnmt3a after Stat5a was knocked down. Full-length blot images are presented in Supplementary Fig. . D. In wild type BaF3 cells, the protein expression level of Dnmt3a after overexpression of Stat5a. Full-length blot images are presented in Supplementary Fig. . E & F. In <t>Jak2</t> <t>V617F</t> BaF3 cells, the protein expression of Stat5a after knockdown or overexpression of Dnmt3a. Full-length blot images are presented in Supplementary Fig. and Supplementary Fig. . G & H. In wild-type BaF3 cells, the protein expression of Stat5a after knockdown or overexpression of Dnmt3a. Full-length blot images are presented in Supplementary Fig. and Supplementary Fig.
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    primary monoclonal antibodies against jak2  (Cell Signaling Technology Inc)
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    Dnmt3a expression is repressed by Stat5a and Stat5a is the upstream regulator of Dnmt3a. A. The expression levels of p-STAT5a and DNMT3a proteins in human myeloid tumor cell lines. Full-length blot images are presented in Supplementary Fig. . B. The expression levels of p-Stat5a and Dnmt3a proteins in murine myeloid tumor cell lines. Full-length blot images are presented in Supplementary Fig. . C. In wild type BaF3 cells, the protein expression level of Dnmt3a after Stat5a was knocked down. Full-length blot images are presented in Supplementary Fig. . D. In wild type BaF3 cells, the protein expression level of Dnmt3a after overexpression of Stat5a. Full-length blot images are presented in Supplementary Fig. . E & F. In <t>Jak2</t> <t>V617F</t> BaF3 cells, the protein expression of Stat5a after knockdown or overexpression of Dnmt3a. Full-length blot images are presented in Supplementary Fig. and Supplementary Fig. . G & H. In wild-type BaF3 cells, the protein expression of Stat5a after knockdown or overexpression of Dnmt3a. Full-length blot images are presented in Supplementary Fig. and Supplementary Fig.
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    Dnmt3a expression is repressed by Stat5a and Stat5a is the upstream regulator of Dnmt3a. A. The expression levels of p-STAT5a and DNMT3a proteins in human myeloid tumor cell lines. Full-length blot images are presented in Supplementary Fig. . B. The expression levels of p-Stat5a and Dnmt3a proteins in murine myeloid tumor cell lines. Full-length blot images are presented in Supplementary Fig. . C. In wild type BaF3 cells, the protein expression level of Dnmt3a after Stat5a was knocked down. Full-length blot images are presented in Supplementary Fig. . D. In wild type BaF3 cells, the protein expression level of Dnmt3a after overexpression of Stat5a. Full-length blot images are presented in Supplementary Fig. . E & F. In <t>Jak2</t> <t>V617F</t> BaF3 cells, the protein expression of Stat5a after knockdown or overexpression of Dnmt3a. Full-length blot images are presented in Supplementary Fig. and Supplementary Fig. . G & H. In wild-type BaF3 cells, the protein expression of Stat5a after knockdown or overexpression of Dnmt3a. Full-length blot images are presented in Supplementary Fig. and Supplementary Fig.
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    Dnmt3a expression is repressed by Stat5a and Stat5a is the upstream regulator of Dnmt3a. A. The expression levels of p-STAT5a and DNMT3a proteins in human myeloid tumor cell lines. Full-length blot images are presented in Supplementary Fig. . B. The expression levels of p-Stat5a and Dnmt3a proteins in murine myeloid tumor cell lines. Full-length blot images are presented in Supplementary Fig. . C. In wild type BaF3 cells, the protein expression level of Dnmt3a after Stat5a was knocked down. Full-length blot images are presented in Supplementary Fig. . D. In wild type BaF3 cells, the protein expression level of Dnmt3a after overexpression of Stat5a. Full-length blot images are presented in Supplementary Fig. . E & F. In Jak2 V617F BaF3 cells, the protein expression of Stat5a after knockdown or overexpression of Dnmt3a. Full-length blot images are presented in Supplementary Fig. and Supplementary Fig. . G & H. In wild-type BaF3 cells, the protein expression of Stat5a after knockdown or overexpression of Dnmt3a. Full-length blot images are presented in Supplementary Fig. and Supplementary Fig.

    Journal: BMC Cancer

    Article Title: Dnmt3a is downregulated by Stat5a and mediates G0/G1 arrest by suppressing the miR-17-5p/Cdkn1a axis in Jak2 V617F cells

    doi: 10.1186/s12885-021-08915-0

    Figure Lengend Snippet: Dnmt3a expression is repressed by Stat5a and Stat5a is the upstream regulator of Dnmt3a. A. The expression levels of p-STAT5a and DNMT3a proteins in human myeloid tumor cell lines. Full-length blot images are presented in Supplementary Fig. . B. The expression levels of p-Stat5a and Dnmt3a proteins in murine myeloid tumor cell lines. Full-length blot images are presented in Supplementary Fig. . C. In wild type BaF3 cells, the protein expression level of Dnmt3a after Stat5a was knocked down. Full-length blot images are presented in Supplementary Fig. . D. In wild type BaF3 cells, the protein expression level of Dnmt3a after overexpression of Stat5a. Full-length blot images are presented in Supplementary Fig. . E & F. In Jak2 V617F BaF3 cells, the protein expression of Stat5a after knockdown or overexpression of Dnmt3a. Full-length blot images are presented in Supplementary Fig. and Supplementary Fig. . G & H. In wild-type BaF3 cells, the protein expression of Stat5a after knockdown or overexpression of Dnmt3a. Full-length blot images are presented in Supplementary Fig. and Supplementary Fig.

    Article Snippet: Primary antibodies against Jak2 (cat. no. 3230), p-Jak2 (cat. no. 3771), Stat5a (cat. no. 94205), p-Stat5a (cat. no. 4322), Dnmt3a (cat. no. 3598), β-actin (cat. no. 8457) and Cdkn1a (cat. no. 64016) were purchased from Cell Signaling Technology (USA).

    Techniques: Expressing, Over Expression, Knockdown

    Knockdown or overexpression of Dnmt3a in Jak2 V617F BaF3 cells significantly affects cell proliferation capacity. A. Soft agar clone formation of Jak2 V617F BaF3 cells; B. Soft agar clone formation of Dnmt3a-overexpressing Jak2 V617F BaF3 cells; C. Soft agar clone formation of Dnmt3a-knockdown Jak2 V617F BaF3 cells; D. Cell proliferation level of Jak2 V617F BaF3 cells with Dnmt3a overexpression or with Dnmt3a knockdown

    Journal: BMC Cancer

    Article Title: Dnmt3a is downregulated by Stat5a and mediates G0/G1 arrest by suppressing the miR-17-5p/Cdkn1a axis in Jak2 V617F cells

    doi: 10.1186/s12885-021-08915-0

    Figure Lengend Snippet: Knockdown or overexpression of Dnmt3a in Jak2 V617F BaF3 cells significantly affects cell proliferation capacity. A. Soft agar clone formation of Jak2 V617F BaF3 cells; B. Soft agar clone formation of Dnmt3a-overexpressing Jak2 V617F BaF3 cells; C. Soft agar clone formation of Dnmt3a-knockdown Jak2 V617F BaF3 cells; D. Cell proliferation level of Jak2 V617F BaF3 cells with Dnmt3a overexpression or with Dnmt3a knockdown

    Article Snippet: Primary antibodies against Jak2 (cat. no. 3230), p-Jak2 (cat. no. 3771), Stat5a (cat. no. 94205), p-Stat5a (cat. no. 4322), Dnmt3a (cat. no. 3598), β-actin (cat. no. 8457) and Cdkn1a (cat. no. 64016) were purchased from Cell Signaling Technology (USA).

    Techniques: Knockdown, Over Expression

    Dnmt3a regulates the cell cycle of Jak2 V617F BaF3 cells by regulating Cdkn1a expression. A. Cell cycle distribution of Jak2 V617F BaF3 cells. B. Cell cycle distribution of Dnmt3a-knockdown Jak2 V617F BaF3 cells. C. Cell cycle distribution of Dnmt3a-overexpressing Jak2 V617F BaF3 cells. D. Cell cycle distribution statistics of each group (A-C) of Jak2 V617F BaF3 cells

    Journal: BMC Cancer

    Article Title: Dnmt3a is downregulated by Stat5a and mediates G0/G1 arrest by suppressing the miR-17-5p/Cdkn1a axis in Jak2 V617F cells

    doi: 10.1186/s12885-021-08915-0

    Figure Lengend Snippet: Dnmt3a regulates the cell cycle of Jak2 V617F BaF3 cells by regulating Cdkn1a expression. A. Cell cycle distribution of Jak2 V617F BaF3 cells. B. Cell cycle distribution of Dnmt3a-knockdown Jak2 V617F BaF3 cells. C. Cell cycle distribution of Dnmt3a-overexpressing Jak2 V617F BaF3 cells. D. Cell cycle distribution statistics of each group (A-C) of Jak2 V617F BaF3 cells

    Article Snippet: Primary antibodies against Jak2 (cat. no. 3230), p-Jak2 (cat. no. 3771), Stat5a (cat. no. 94205), p-Stat5a (cat. no. 4322), Dnmt3a (cat. no. 3598), β-actin (cat. no. 8457) and Cdkn1a (cat. no. 64016) were purchased from Cell Signaling Technology (USA).

    Techniques: Expressing, Knockdown

    The expression level of Cdkn1a is downregulated in Jak2 V617F cells. A. The expression level of Cdkn1a was significantly suppressed in Jak2 V617F BaF3 cells compared with control BaF3 cells. Full-length blot images are presented in Supplementary Fig. . B. The transcription level of Cdkn1a was significantly suppressed in Jak2 V617F BaF3 cells compared with control BaF3 cells. C. The transcription level of Cdkn1a is lower in JAK2 V617F cMPN patients than that in normal controls

    Journal: BMC Cancer

    Article Title: Dnmt3a is downregulated by Stat5a and mediates G0/G1 arrest by suppressing the miR-17-5p/Cdkn1a axis in Jak2 V617F cells

    doi: 10.1186/s12885-021-08915-0

    Figure Lengend Snippet: The expression level of Cdkn1a is downregulated in Jak2 V617F cells. A. The expression level of Cdkn1a was significantly suppressed in Jak2 V617F BaF3 cells compared with control BaF3 cells. Full-length blot images are presented in Supplementary Fig. . B. The transcription level of Cdkn1a was significantly suppressed in Jak2 V617F BaF3 cells compared with control BaF3 cells. C. The transcription level of Cdkn1a is lower in JAK2 V617F cMPN patients than that in normal controls

    Article Snippet: Primary antibodies against Jak2 (cat. no. 3230), p-Jak2 (cat. no. 3771), Stat5a (cat. no. 94205), p-Stat5a (cat. no. 4322), Dnmt3a (cat. no. 3598), β-actin (cat. no. 8457) and Cdkn1a (cat. no. 64016) were purchased from Cell Signaling Technology (USA).

    Techniques: Expressing, Control

    Dnmt3a regulates the cell cycle of Jak2 V617F Baf3 cells through Cdkn1a. A & B. Dnmt3a induced Cdkn1a expression in Jak2 V617F BaF3 cells. Full-length blot images are presented in Supplementary Fig. and Supplementary Fig. . Inhibitor: LY2784544 incubation. C. Jak2 V617F Baf3 cells with Dnmt3a overexpression and with both Dnmt3a overexpression and Cdkn1a knockdown were established. Full-length blot images are presented in Supplementary Fig. . D. Cell cycle distribution of wild-type BaF3 cells. E. Cell cycle distribution of Jak2 V617F BaF3 cells. F. Cell cycle distribution of Jak2 V617F BaF3 cells with Dnmt3a overexpression. G. Cell cycle distribution of Jak2 V617F BaF3 cells with both Dnmt3a overexpression and Cdkn1a knockdown. H. Cell cycle distribution statistics of each group of Jak2 V617F BaF3 cells

    Journal: BMC Cancer

    Article Title: Dnmt3a is downregulated by Stat5a and mediates G0/G1 arrest by suppressing the miR-17-5p/Cdkn1a axis in Jak2 V617F cells

    doi: 10.1186/s12885-021-08915-0

    Figure Lengend Snippet: Dnmt3a regulates the cell cycle of Jak2 V617F Baf3 cells through Cdkn1a. A & B. Dnmt3a induced Cdkn1a expression in Jak2 V617F BaF3 cells. Full-length blot images are presented in Supplementary Fig. and Supplementary Fig. . Inhibitor: LY2784544 incubation. C. Jak2 V617F Baf3 cells with Dnmt3a overexpression and with both Dnmt3a overexpression and Cdkn1a knockdown were established. Full-length blot images are presented in Supplementary Fig. . D. Cell cycle distribution of wild-type BaF3 cells. E. Cell cycle distribution of Jak2 V617F BaF3 cells. F. Cell cycle distribution of Jak2 V617F BaF3 cells with Dnmt3a overexpression. G. Cell cycle distribution of Jak2 V617F BaF3 cells with both Dnmt3a overexpression and Cdkn1a knockdown. H. Cell cycle distribution statistics of each group of Jak2 V617F BaF3 cells

    Article Snippet: Primary antibodies against Jak2 (cat. no. 3230), p-Jak2 (cat. no. 3771), Stat5a (cat. no. 94205), p-Stat5a (cat. no. 4322), Dnmt3a (cat. no. 3598), β-actin (cat. no. 8457) and Cdkn1a (cat. no. 64016) were purchased from Cell Signaling Technology (USA).

    Techniques: Expressing, Incubation, Over Expression, Knockdown

    Dnmt3a negatively regulates miR-17-5p and miR-17-5p represses Cdkn1a expression in Jak2 V617F BaF3 cells. A. The expression level of miR-17-5p in Jak2 V617F BaF3 cells and control BaF3 cells. B. The verification of the knockdown and overexpression effect of Dnmt3a in Jak2 V617F BaF3 cells. Full-length blot images are presented in Supplementary Fig.19. C. The expression level of miR-17-5p in Jak2 V617F BaF3 cells with Dnmt3a knockdown and overexpression. D. The verification of the effect of transfection of miR-17-5p mimic and inhibitor in Jak2 V617F BaF3 cells. E & F. The transcription and expression of Cdkn1a in Jak2 V617F BaF3 cells transfected with miR-17-5p mimic or inhibitor. Full-length blot images are presented in Supplementary Fig.19. G. Graphic abstract of the present study

    Journal: BMC Cancer

    Article Title: Dnmt3a is downregulated by Stat5a and mediates G0/G1 arrest by suppressing the miR-17-5p/Cdkn1a axis in Jak2 V617F cells

    doi: 10.1186/s12885-021-08915-0

    Figure Lengend Snippet: Dnmt3a negatively regulates miR-17-5p and miR-17-5p represses Cdkn1a expression in Jak2 V617F BaF3 cells. A. The expression level of miR-17-5p in Jak2 V617F BaF3 cells and control BaF3 cells. B. The verification of the knockdown and overexpression effect of Dnmt3a in Jak2 V617F BaF3 cells. Full-length blot images are presented in Supplementary Fig.19. C. The expression level of miR-17-5p in Jak2 V617F BaF3 cells with Dnmt3a knockdown and overexpression. D. The verification of the effect of transfection of miR-17-5p mimic and inhibitor in Jak2 V617F BaF3 cells. E & F. The transcription and expression of Cdkn1a in Jak2 V617F BaF3 cells transfected with miR-17-5p mimic or inhibitor. Full-length blot images are presented in Supplementary Fig.19. G. Graphic abstract of the present study

    Article Snippet: Primary antibodies against Jak2 (cat. no. 3230), p-Jak2 (cat. no. 3771), Stat5a (cat. no. 94205), p-Stat5a (cat. no. 4322), Dnmt3a (cat. no. 3598), β-actin (cat. no. 8457) and Cdkn1a (cat. no. 64016) were purchased from Cell Signaling Technology (USA).

    Techniques: Expressing, Control, Knockdown, Over Expression, Transfection

    Aberrant activation of the JAK-STAT pathway represses Dnmt3a transcription and translation via Stat5a. A. The expression level of Jak2, p-Jak2, Stat5a, p-Stat5a and Dnmt3a in wild-type and Jak2 V617F BaF3 cells. Full-length blot images are presented in Supplementary Fig. . B. Transcription level of DNMT3a in bone marrow mononuclear cells from JAK2 V617F MPN patients and normal controls. C. p-Stat5a and Dnmt3a of Jak2 V617F Baf3 cells incubated with AZ960. Full-length blot images are presented in Supplementary Fig. . D. p-Stat5a and Dnmt3a of Jak2 V617F Baf3 cells incubated with LY2784544. Full-length blot images are presented in Supplementary Fig. . E. In Jak2 V617F BaF3 cells, the protein expression level of Dnmt3a after Stat5a was knocked down. Full-length blot images are presented in Supplementary Fig. . F. The transcriptional level of Dnmt3a in Jak2 V617F Baf3 cells with or without LY2784544 incubation

    Journal: BMC Cancer

    Article Title: Dnmt3a is downregulated by Stat5a and mediates G0/G1 arrest by suppressing the miR-17-5p/Cdkn1a axis in Jak2 V617F cells

    doi: 10.1186/s12885-021-08915-0

    Figure Lengend Snippet: Aberrant activation of the JAK-STAT pathway represses Dnmt3a transcription and translation via Stat5a. A. The expression level of Jak2, p-Jak2, Stat5a, p-Stat5a and Dnmt3a in wild-type and Jak2 V617F BaF3 cells. Full-length blot images are presented in Supplementary Fig. . B. Transcription level of DNMT3a in bone marrow mononuclear cells from JAK2 V617F MPN patients and normal controls. C. p-Stat5a and Dnmt3a of Jak2 V617F Baf3 cells incubated with AZ960. Full-length blot images are presented in Supplementary Fig. . D. p-Stat5a and Dnmt3a of Jak2 V617F Baf3 cells incubated with LY2784544. Full-length blot images are presented in Supplementary Fig. . E. In Jak2 V617F BaF3 cells, the protein expression level of Dnmt3a after Stat5a was knocked down. Full-length blot images are presented in Supplementary Fig. . F. The transcriptional level of Dnmt3a in Jak2 V617F Baf3 cells with or without LY2784544 incubation

    Article Snippet: Primary antibodies against Jak2 (cat. no. 3230), p-Jak2 (cat. no. 3771), Stat5a (cat. no. 94205), p-Stat5a (cat. no. 4322), Dnmt3a (cat. no. 3598), β-actin (cat. no. 8457) and Cdkn1a (cat. no. 64016) were purchased from Cell Signaling Technology (USA).

    Techniques: Activation Assay, Expressing, Incubation